In order to better understand what mediating capabilities are needed to use B2 B technology to improve supply chain performance(SCP)and what relationships exist among these capabilities，grounded in the IT process-oriented view and the capability hierarchy view，this paper identified three different levels of mediating capabilities，i.e.，application capability of e-business(ACEB)，e-business systems capability(EBSC)，and e-business support capability for collaborative strategy(ESCS)，and then developed a theoretical model for addressing the impacts of ACEB on the EBSC and ESCS．A confirmatory factor analysis Writ8 performed to test this model by using 1 52 valid samples from Chinese manufacturing sectors．The results validate the multi-dimension constructs of the ACEB，and show that the ACEB have a direct effect on the EBSC，a direct and indirect effect on the ESCS，and a moderating effect on the relationship between the EBSC and the ESCS．