Traditional analyses of industrial and supply chain ecosystems have predominantly focused on supply relationships involving products and services. However, inter-firm connections extend beyond supply relationships to include equity investments and technological collaborations. Ownership penetration network analysis helps assess the interests represented by enterprises, as well as the stability and reliability of their supplies. Meanwhile, an analysis of technological networks allows for a forward-looking evaluation of a firm’s technological potential and substitutability. To enable a more comprehensive analysis of industrial and supply chain ecosystems, this paper proposes the SPOT framework, which integrates four types of networks: supply relationship, product, ownership, and technology networks, to guide the construction and analysis of such ecosystems. While existing research and applications often rely on a single network, the SPOT framework emphasizes the interconnection and integrated analysis of these four networks. This paper elaborates on the data sources and construction methods required for building the SPOT framework, along with its applications at both the macro-meso levels and the individual enterprise level. Moreover, it discusses the implications of the SPOT framework for academic research.